Phospho-p70 S6 Kinase 1-T389 Polyclonal Antibody-BS79403

Phospho-p70 S6 Kinase 1-T389 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79403-50, BS79403-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P23443

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 70KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NIH/3T3, using Phospho-p70 S6 Kinase 1-T389 antibody at 1:500 dilution.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s., Immunofluorescence analysis of C6 cells using Phospho-p70 S6 Kinase 1-T389 antibody at dilution of 1:100. C6 cells were treated by Serum-starvation overnight at 37℃., Immunofluorescence analysis of HeLa cells using Phospho-p70 S6 Kinase 1-T389 antibody at dilution of 1:100. HeLa cells were treated by Serum-starvation overnight at 37℃.

Alternative Name: PS6K; S6K; S6K-beta-1; S6K1; STK14A; p70 S6KA; p70(S6K)-alpha; p70-S6K; p70-alpha; P70 S6K; RPS6KB1; p70S6KA

Immunogen: A synthetic phosphorylated peptide around T389 of human P70 S6K(NP_003152.1).

Conjugate: Unconjugated

Modification: Phosphorylated