Phospho-p70 S6 Kinase 1-T421/S424 Polyclonal Antibody-BS79483

Phospho-p70 S6 Kinase 1-T421/S424 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79483-50, BS79483-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P23443

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of NIH/3T3 cells, using Phospho-p70 S6 Kinase 1-T421/S424 pAb at 1:1000 dilution or P70 S6K antibody. NIH/3T3 cells were treated by EGF at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 180s.

Bioworld Molecular Weight: 70kDa

Specificity: Phosphorylated

Alternative Name: PS6K; S6K; S6K-beta-1; S6K1; STK14A; p70 S6KA; p70(S6K)-alpha; p70-S6K; p70-alpha; P70 S6K; RPS6KB1; p70S6KA

Immunogen: A synthetic phosphorylated peptide around T421 & S424 of human RPS6KB1(NP_001258989.1).

Conjugate: Unconjugated

Modification: Phosphorylated