Phospho-p90Rsk/RSK1/RPS6KA1-S363 Polyclonal Antibody-BS79427

Phospho-p90Rsk/RSK1/RPS6KA1-S363 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79427-50, BS79427-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15418

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IP

All Applications: WB,1:500 - 1:2000 | IP,1:50 - 1:100

Background: This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using at 1:1000 dilution. HeLa cells were treated by PMA/TPA at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Bioworld Molecular Weight: 90KDa

Specificity: Phosphorylated

Alternative Name: HU-1; MAPKAPK1A; RSK; RSK1; p90Rsk; RPS6KA1

Immunogen: A synthetic phosphorylated peptide around S363 of human p90Rsk/RSK1/RPS6KA1(NP_002944.2).

Conjugate: Unconjugated

Modification: Phosphorylated