Product Description

Phospho-p90Rsk/RSK1/RPS6KA1-S380 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79402-50, BS79402-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15418

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP,

All Applications: WB,1:500 - 1:2000 | IP,1:50 - 1:100

Background: This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 90kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Phospho-p90Rsk/RSK1/RPS6KA1-S380 antibody at 1:1000 dilution or RPS6KA1 antibody. HeLa cells were treated by PMA/TPA at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s., Western blot analysis of extracts of various cell lines, using Phospho-p90Rsk/RSK1/RPS6KA1-S380 antibody at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-p90Rsk/RSK1/RPS6KA1-S380 pAb. Western blot was performed from the immunoprecipitate using Phospho-p90Rsk/RSK1/RPS6KA1-S380 pAb at a dilution of 1:1000. HeLa cells were treated by EGF at 37℃ for 30 minutes after serum-starvation overnight.

Alternative Name: HU-1; MAPKAPK1A; RSK; RSK1; p90Rsk; RPS6KA1

Immunogen: A synthetic phosphorylated peptide around S380 of human p90Rsk/RSK1/RPS6KA1(NP_002944.2).

Conjugate: Unconjugated

Modification: Phosphorylated