Product Description

Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11368-50, MB11368-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: O75914

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Background: PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. PAK3 forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 62 kDa; Observed MW: 62 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PAK1 /PAK2 /PAK3 in C6, 3T3, Hela lysates using Phospho-PAK1/2/3 antibody., Immunocytochemistry analysis of PAK1 /PAK2 /PAK3 in Hela using PAK1 /PAK2 /PAK3 antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-PAK1/2/3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: PAK3; OPHN3; Serine/threonine-protein kinase PAK 3; Beta-PAK; Oligophrenin-3; p21-activated kinase 3; PAK-3

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser144 of human PAK1

Conjugate: Unconjugated

Modification: Phosphorylated