Phospho-PEA15-S104 Polyclonal Antibody-BS74172

Phospho-PEA15-S104 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74172-50, BS74172-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15121

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB,

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 13kDa/15kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HeLa and C6 cells, using Phospho-PEA15-S104 antibody at 1:2000 dilution. HeLa cells were treated by PMA/TPA for 15 minutes after serum-starvation overnight.C6 cells were treated by PMA/TPA for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 10s., Western blot analysis of extracts of HeLa cells, using Phospho-PEA15-S104 pAb at 1:2000 dilution or PEA15 antibody. HeLa cells were treated by PMA/TPA for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 90s.

Alternative Name: PEA15; HMAT1; HUMMAT1H; MAT1; MAT1H; PEA-15; PED

Immunogen: A synthetic phosphorylated peptide around S104 of human PEA15(NP_003759.1).

Conjugate: Unconjugated

Modification: Phosphorylated