Product Description

Phospho-PERK (Thr980) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65625-50, BS65625-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: Q9Z2B5

Host: Rabbit

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Jan 2010].

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phospho-PERK (Thr980) Polyclonal Antibody detects endogenous levels of Phospho-PERK protein only when phosphorylated at Thr980

Bioworld Molecular Weight: 119kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PERK (Thr980)) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PERK(Thr980)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C

Alternative Name: p-PERK(Thr980); PERK(Phospho Thr980); PERK(Phospho T980); HRI; HsPEK; Pancreatic eIF2-alpha kinase; PEK; PRKR like endoplasmic reticulum kinase; WRS; DKFZp781H1925; EC 2.7.11.1; EIF2AK3; Eukaryotic translation initiation factor 2 alpha kinase 3; Heme regulated EIF2 alpha kinase.

Immunogen: KLH conjugated synthesised phosphopeptide derived from mouse PERK around the phosphorylation site of Thr980:H(p-T)GQ

Conjugate: Unconjugated

Modification: Phosphorylation