Product Description

Phospho-PERK (Thr982) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BZ16392-50, BZ16392-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: ICC/IF, WB, IHC-F, IHC-P, ELISA

Alternate Names: EIF2AK3, PEK, PERK, Eukaryotic translation initiation factor 2-alpha kinase 3, PRKR-like endoplasmic reticμlum kinase, Pancreatic eIF2-alpha kinase, HsPEK

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: The antiserum was produced against synthesized peptide derived from human PEK/PERK around the phosphorylation site of Thr982. AA range:947-996

Dilution: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Purification: Affinity Purified

Specificity: IgG

Molecular Weight: Calcμlated MW: 125 kDa, Observed MW: 130 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9NZJ5

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only