Phospho-PI 3 kinase p85 alpha /gamma (Tyr467/199) Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-20966-60, E-AB-20966-120, E-AB-20966-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: PI 3-kinase p85/p55 (phospho Tyr467/199)
Target Synonym: GRB1; p85 alpha; p85; P85A; Phosphatidylinositol 3 kinase associated p 85 alpha; Phosphatidylinositol 3 kinase regulatory 1; Phosphatidylinositol 3 kinase; regulatory subunit; polypeptide 1 (p85 alpha); Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Phosphatidylinositol 3-kinase regulatory subunit alpha; Phosphoinositide 3 kinase; regulatory subunit 1 (alpha); PI3 kinase p85 subunit alpha; PI3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K; PI3K regulatory subunit alpha; Pik3r1; PtdIns 3 kinase p85 alpha; PtdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory subunit p85-alpha
Research Areas: Cancer, Immunology, Signal Transduction
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat, Monkey
Application: WB, IHC-p, IF, ELISA
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: P27986,Q92569
Background: The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
Concentration: 1 mg/mL
Immunogen: Synthesized peptide derived from human PI 3-kinase p85/p55 around the phosphorylation site of Tyr467/199
Buffer: PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:10000
Calculated MW: 54+83kDa
Observed MW: 55+85kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only