Phospho PKC θ (Y90) rabbit pAb - ES20173

Phospho PKC θ (Y90) rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES20173-50, ES20173-100

Citations, Manuals and MSDS Available upon request.

Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein., cofactor:Magnesium., domain:The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain., enzyme regulation:Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation., function:PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters., function:This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production., PTM:Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation., similarity:Belongs to the protein kinase superfamily., similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily., similarity:Contains 1 AGC-kinase C-terminal domain., similarity:Contains 1 C2 domain., similarity:Contains 1 protein kinase domain., similarity:Contains 2 phorbol-ester/DAG-type zinc fingers., subunit:Interacts with TXNL2/PICOT., tissue specificity:Skeletal muscle, megakaryoblastic cells and platelets.,

Alternate Name: Protein kinase C theta type (EC 2.7.11.13; nPKC-theta)

Source: Rabbit

Applications: WB; IHC

Dilution: WB 1:500-2000; IHC-p 1:50-300

Reactivity: Human; Mouse; Rat

Immunogen: Synthesized peptide derived from human Phospho PKC θ (Y90)

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 78kD

Human Gene ID: 5588

Human SWISS Prot NO: Q04759

Subcellular Location: Cytoplasm. Cell membrane; Peripheral membrane protein. In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.

Research Use Only