Phospho-PKC zeta-T410 Polyclonal Antibody-BS74144

Phospho-PKC zeta-T410 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74144-50, BS74144-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q05513

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

All Applications: IHC,1:50 - 1:100

Background: Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Immunogen: A phospho specific peptide corresponding to residues surrounding T410 of human PKC (pan)(zeta).

Conjugate: Unconjugated

Modification: Phosphorylated

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry of paraffin-embedded human lung carcinoma tissue, using Phospho-PRKCZ-T410 antibody.Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.