Phospho-PKCalpha/beta II-T638/641 Polyclonal Antibody-BS74022

Phospho-PKCalpha/beta II-T638/641 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74022-50, BS74022-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P17252/P05771

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:100 | IF/ICC,1:100 - 1:200

Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated Antibodies

Bioworld Molecular Weight: 80kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PKCalpha/beta II-T638/641 pAb at 1:1000 dilution or PRKCA antibody. NIH/3T3 cells were treated by PMA/TPA for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunohistochemistry of paraffin-embedded human lung carcinoma tissue, using Phospho-PKCalpha/beta II-T638/641 antibody. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunofluorescence analysis of methanol-fixed MCF-7 cells using Phospho-PRKCB-T641 antibody.

Alternative Name: PKC-beta; PKCB; PRKCB1; PRKCB2

Immunogen: A synthetic phosphorylated peptide around T638 of human PKCalpha/beta IIPRKCA(NP_002728.2).

Conjugate: Unconjugated

Modification: Phosphorylated