Product Description

Phospho-PKR (Thr446) Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS65508-50, BS65508-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: PKR is an interferon-inducible serine/threonine specific protein kinase. It is widely expressed in eukaryotic organisms and activated by double stranded RNA. Activation of PKR by dsRNAs leads to autophosphorylation at mμltiple sites. Phosphorylation of Thr446 and Thr451 in the PKR activation loop is required in vivo and in vitro for high level kinase activity. PKR phosphorylates its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor 2 (EIF2 alpha), leading to the inhibition of protein synthesis. PKR is also involved in TLR signaling and mediates apoptosis in fibroblasts in response to viral infection and inflammatory cytokines, and also activates IKK and NFKB, thereby suppressing apoptosis. Recently, it has been reported that PKR also phosphorylates human p53 on serine 392. PKR might play a role in ER stress-induced apoptosis and in Alzheimer's disease. Alzheimer cases show prominent PKR activation in association with neuritic plaques and pyramidal neurons in the hippocampus and neocortex.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC-P

Alternate Names: PKR(T446), PKR (phospho T446), p-PKR (phospho T446), PKR(Phospho-Thr446), double-stranded RNA-dependent Protein Kinase, interferon-induced, double-stranded RNA-activated protein kinase isoform a, protein kinase, interferon-inducible double stranded RNA dependent, interferon-inducible elF2alpha kinase, double stranded RNA activated protein kinase, p68 kinase, eIF-2A protein kinase 2, P1/eIF-2A protein kinase, protein kinase RNA-activated, interferon-inducible RNA-dependent protein kinase, EIF2AK2, EIF2AK1, MGC126524, PKR, PRKRv, E2AK2_HUMAN.

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PKR around the phosphorylation site of Thr446:KR(p-T)RS

Dilution: IHC-P=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations shoμld be determined by the end user.

Purification: affinity purified by Protein A

Specificity: Phospho-PKR (Thr446) monoclonal antibody detects endogenous levels of Nrf2 protein only when phosphorylated at Thr446.

Molecular Weight: ~67kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q52M43

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Research Use Only