Product Description

Phospho-Progesterone Receptor(Ser190) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65628-50, BS65628-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: Q63449

Host: Rabbit

Reactivity: Mouse, Rat, Cow,

Applications: WB,

All Applications: WB=1:500-2000

Background: Estrogen and progesterone receptor are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements(EREs) alters transcription of ERE-containing genes. The carboxy terminal region of the estrgen receptor contains the ligand binding domain, the amino terminus serves as the transactivation domain, and the DNA binding domain is centrally located. Two forms of estrogen receptor have been identified, ER alpha and ER beta. ER alpha and ER beta have been shown to be differentially activated by various ligands. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-Aand hPR-B), which arise from alternative splicing. In most cells, hPR-B functions as a transcriptional activator of progesterone-responsive gene, whereas hPR-A function as a transcriptional inhibitor of all steroid hormone receptors.

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phospho-Progesterone Receptor (Ser190) Polyclonal Antibody detects endogenous levels of Phospho-Progesterone Receptor protein only when phosphorylated at Ser190

Bioworld Molecular Weight: 110 kD/ 120kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Sample:
Lane 1: Uterus (Mouse) Lysate at 40 ug
Lane 2: Testis (Mouse) Lysate at 40 ug
Lane 3: Testis (Rat) Lysate at 40 ug
Lane 4: MCF-7 (Human) Cell Lysate at 30 ug
Lane 5: HepG2 (Human) Cell Lysate at 30 ug
Lane 6: Hela (Human) Cell Lysate at 30 ug
Primary: Anti-progesterone receptor at 1/1000 dilution, Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 ug
Lane 2: Uterus (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-Progesterone Receptor (Ser190) at 1/1000 dilution

Alternative Name: PGR(phospho S190); NR3C3; Nuclear receptor subfamily 3 group C member 3; PGR; PR; PRA; PRB; Progesterone receptor; Progestin receptor form A; Progestin receptor form B; PRGR_RAT.

Immunogen: KLH conjugated Synthesised phosphopeptide derived from rat Progesterone Receptor around the phosphorylation site of Ser190

Conjugate: Unconjugated

Modification: Phosphorylation