Phospho-PTEN-S380/T382/383 Polyclonal Antibody-BS79503

Phospho-PTEN-S380/T382/383 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79503-50, BS79503-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P60484

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

All Applications: IHC,1:50 - 1:200

Background: This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-PTEN-S380/T382/383 Rabbit pAb at dilution of 1:200. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse heart using Phospho-PTEN-S380/T382/383 Rabbit pAb at dilution of 1:200. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded rat lung using Phospho-PTEN-S380/T382/383 Rabbit pAb at dilution of 1:200. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Alternative Name: 10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; TEP1; PTEN; PTENbeta

Immunogen: A synthetic phosphorylated peptide around S380 & T382 & T383 of human PTEN(NP_000305.3).

Conjugate: Unconjugated

Modification: Phosphorylated