Product Description

Phospho-Raf1-S259 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79384-50, BS79384-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04049

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IP,

All Applications: WB,1:500 - 1:2000 | IP,1:50 - 1:100

Background: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 73kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HeLa cells, using Phospho-Raf1-S259 antibody at 1:1000 dilution. HeLa cells were treated by 10% FBS after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA., Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-Raf1-S259 pAb. Western blot was performed from the immunoprecipitate using Phospho-Raf1-S259 pAb at a dilution of 1:1000. HeLa cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.

Alternative Name: CMD1NN; CRAF; NS5; Raf-1; c-Raf; RAF1

Immunogen: A synthetic phosphorylated peptide around S259 of human Phospho-Raf1-S259(NP_002871.1).

Conjugate: Unconjugated

Modification: Phosphorylated