Phospho-Raf1-S338 Polyclonal Antibody-BS79385

Phospho-Raf1-S338 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79385-50, BS79385-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04049

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Raf1-S338 pAb at 1:1000 dilution or Phospho-Raf1-S338 antibody. NIH/3T3 cells were treated by PMA/TPA for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5s.

Bioworld Molecular Weight: 73kDa

Specificity: Phosphorylated

Alternative Name: CMD1NN; CRAF; NS5; Raf-1; c-Raf; RAF1

Immunogen: A synthetic phosphorylated peptide around S338 of human Phospho-Raf1-S338(NP_002871.1).

Conjugate: Unconjugated

Modification: Phosphorylated