Product Description

Phospho-Raf1 (Ser259) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16727-50, BZ16727-100

Product: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Swiss-Prot: P04049

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB,IHC-P

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100

Background: Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-Raf1 in 293TLysates; 293TLysates treated with Okadaic Acid + Calyculin A using Phospho-Raf1 antibody.

Bioworld Molecular Weight: Calculated MW: 73 kDa; Observed MW: 73 kDa

Specificity: IgG

Alternative Name: RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

Immunogen: A synthesized peptide derived from human Phospho-Raf1 (S259)

Conjugate: Unconjugated

Modification: Phosphorylated