Phospho-Rb2 p130 (Ser952) Monoclonal Antibody-MB11522

Phospho-Rb2 p130 (Ser952) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11522-50, MB11522-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q08999

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-F, IHC-P, ICC/IF

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 128 kDa; Observed MW: 128 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Rb2 p130 in K562, Hela lysates using Phospho-Rb2 p130 antibody., Immunocytochemistry analysis of Rb2 p130 in Hela using Rb2 p130 antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human tonsil using Rb2 p130 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: Rb2; P130

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser952 of human Rb2 p130

Conjugate: Unconjugated

Modification: Phosphorylated