Phospho-RIPK3-S232 Polyclonal Antibody-BS74215

Phospho-RIPK3-S232 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74215-50, BS74215-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9QZL0

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB, 1:500 - 1:2000

Background: The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 53KDa

Immunogen: A phospho specific peptide corresponding to residues surrounding S232 of MouseRipk3.

Conjugate: Unconjugated

Modification: Phosphorylated

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of 293T cells, using Phospho-RIPK3-S232 antibody at 1:500 dilution.293T cells were treated by CIP at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.