Product Description

Phospho-RPS6KA5-T581 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS74220-50, BS74220-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regμlation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regμlation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimμli such as UV-C irradiation, epidermal growth factor (EGF and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress. In erythropoietin-stimμlated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regμlation of its transcriptional potential. Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regμlates its ability to stimμlate transcription, which may be important during development and breast tumor formation. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimμli and EGF, which resμlts in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: RPS6KA5, MSK1, MSPK1, RLPK

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: A synthetic phosphorylated peptide around T581 of human RPS6KA5(NP_004746.2).

Dilution: WB, 1:500 - 1:2000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Phosphorylated

Molecular Weight: Refer to figures

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O75582

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only