Phospho-Smad2-S465/467 + Smad3-S423/425 Polyclonal Antibody-BS79400

Phospho-Smad2-S465/467 + Smad3-S423/425 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS79400-50, BS79400-100 Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: Q15796/P84022 Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB, IHC, IF/ICC All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200 Background: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012] Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Phosphorylated Bioworld Molecular Weight: 60KDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of C2C12, using Phospho-Smad2-S465/467+Smad3-S423/425 antibody at 1:890 dilution.C2C12 cells were treated by TGF-β at 37℃ for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per Lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s., Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human esophageal cancer using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse stomach using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol. Alternative Name: HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3 Immunogen: A synthetic phosphorylated peptide around S465 & S467 of human Phospho-Smad2-S465/467 + Smad3-S423/425SMAD2(NP_005892.1). Conjugate: Unconjugated Modification: Phosphorylated