Product Description

Phospho-Smad2-T220 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS79456-50, BS79456-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modμlators that mediate mμltiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regμlates mμltiple cellμlar processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC

Alternate Names: JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2, Smad2, SMAD2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: A synthetic phosphorylated peptide around T220 of human Smad2(NP_005892.1).

Dilution: IHC, 1:50 - 1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Phosphorylated

Molecular Weight: Refer to figures

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q15796

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only