Product Description

Phospho-Src (Tyr419) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16612-50, BZ16612-100

Product: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Swiss-Prot: P12931

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB,FC

All Applications: WB: 1/500-1/1000 FC: 1/50-1/100

Background: Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-Src in A431Lysates treated with pervanadate using Phospho-Src antibody.

Bioworld Molecular Weight: Calculated MW: 60 kDa; Observed MW: 60 kDa

Specificity: IgG

Alternative Name: SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src

Immunogen: A synthesized peptide derived from human Phospho-Src (Y419)

Conjugate: Unconjugated

Modification: Phosphorylated