Product Description

Phospho-Src (Tyr530) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11896-50, MB11896-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P12931

Host: Rabbit

Reactivity: Rat

Applications: WB, IP,

All Applications: WB: 1/500-1/1000 IP: 1/20

Background: Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 60 kDa; Observed MW: 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-Src in rat lung lysates using Phospho-Src antibody., Western blot analysis of Src in CHO-K1 lysates using Src antibody.

Alternative Name: SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Tyr529 of human Src

Conjugate: Unconjugated

Modification: Phosphorylated