Phospho-TAK1-S412 Polyclonal Antibody-BS6397

Phospho-TAK1-S412 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6397-50, BS6397-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43318

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB,

All Applications: WB,1:500 - 1:2000

Background: The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated Antibodies

Bioworld Molecular Weight: 75kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Phospho-TAK1-S412 Rabbit pAb at 1:1000 dilution.293 cells were treated by Calyculin A at 37℃ for 30 minutes after serum-starvation overnight.K-562 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.,
Western blot analysis of extracts of various cell lines, using Phospho-TAK1-S412 antibody at 1:1000 dilution. C2C12 cells were treated by TGF-β at 37℃ for 30 minutes.C6 cells were treated by Calyculin A at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 120s.

Alternative Name: CSCF; FMD2; MEKK7; TAK1; TGF1a; MAP3K7

Immunogen: A synthetic phosphorylated peptide around S412 of human TAK1(NP_003179.1).

Conjugate: Unconjugated

Modification: Phosphorylated