Phospho-TrkA-Y674/675 + TrkB-Y706/707 Polyclonal Antibody-BS74152

Phospho-TrkA-Y674/675 + TrkB-Y706/707 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74152-50, BS74152-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04629/Q16620

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008]

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HeLa cells, using Phospho-TrkA-Y674/675 + TrkB-Y706/707 antibody at 1:500 dilution. HeLa cells were treated by Pervanadate at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Bioworld Molecular Weight: 140KDa

Specificity: Phosphorylated

Alternative Name: NTRK1/NTRK2

Immunogen: A synthetic phosphorylated peptide around Y674 & Y675 of human TrkANTRK1(NP_001012331.1).

Conjugate: Unconjugated

Modification: Phosphorylated