Phospho-ZAP70-Y493 Polyclonal Antibody-BS79376

Phospho-ZAP70-Y493 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79376-50, BS79376-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P43403

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:2000 - 1:5000 | IHC,1:50 - 1:100 | IF/ICC,1:50 - 1:200

Background: This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 70KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of Jurkat cells, using Phospho-ZAP70-Y493 antibody at 1:1000 dilution.Jurkat cells were treated by Hydrogen Peroxide at 37℃ for 2 minutes after serum-starvation overnight.Jurkat cells were treated by Pervanadate at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-ZAP70-Y493 antibody. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunofluorescence analysis of Jurkat cells using Phospho-ZAP70-Y493 Rabbit pAb at dilution of 1:100.Jurkat cells were treated by Hydrogen Peroxide at 37℃ for 2 minutes after serum-starvation overnight. Blue: DAPI for nuclear staining.

Alternative Name: ZAP70; ADMIO2; IMD48; SRK; STCD; STD; TZK; ZAP-70

Immunogen: A synthetic phosphorylated peptide around Y493 of human ZAP70(NP_001070.2).

Conjugate: Unconjugated

Modification: Phosphorylated