Product Description

PI3K p101 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS62421-50, BS62421-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3, 4, 5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4, 5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival. PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN. PI3Ks are composed of a catalytic subunit (p110) and a regμlatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regμlatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β. In contrast, p110γ associates with a p101 regμlatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins. The p101 regμlatory subunit binds to Gβγ, released from heterotrimeric G proteins, and recruits bound p110γ catalytic subunit to the plasma membrane, which is required for GPCR-induced PI3Kγ activity.

Category: Primary Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: WB, IHC

Alternate Names: Phosphoinositide 3-kinase regμlatory subunit 5, PI3-kinase regμlatory subunit 5, PI3-kinase p101 subunit, Phosphatidylinositol 4, 5-bisphosphate 3-kinase regμlatory subunit, PtdIns-3-kinase regμlatory subunit, Protein FOAP-2, PtdIns-3-kinase p101, p101-PI3K, PIK3R5

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to Human PI3K p101.

Dilution: WB:1:500~1:1000 IHC:1:50~1:200

Purification: The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE).

Specificity: PI3K p101 polyclonal antibody detects endogenous levels of PI3K p101 protein.

Molecular Weight: ~ 101 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q8WYR1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Research Use Only