Product Description

PI3K p85α/p55γ (Y467/199) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: AP0230-50, AP0230-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regμlatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecμle that regμlates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insμlin-stimμlated increase in glucose uptake and glycogen synthesis in insμlin-sensitive tissues.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Phosphatidylinositol 3-kinase regμlatory subunit alpha, PI3-kinase regμlatory subunit alpha, PI3K regμlatory subunit alpha, PtdIns-3-kinase regμlatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regμlatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regμlatory subunit p85-alpha, PIK3R1, GRB1, Phosphatidylinositol 3-kinase regμlatory subunit gamma, PI3-kinase regμlatory subunit gamma, PI3K regμlatory subunit gamma, PtdIns-3-kinase regμlatory subunit gamma, Phosphatidylinositol 3-kinase 55 kDa regμlatory subunit gamma, PI3-kinase subunit p55-gamma, PtdIns-3-kinase regμlatory subunit p55-gamma, p55PIK, PIK3R3

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Synthetic peptide, corresponding to amino acids 430-480 of Human PI3K p85α.

Dilution: WB: 1:500~1:1000
IHC:1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PI3K p85α/p55γ (Y467/199) polyclonal antibody detects endogenous levels of PI3-kinase p85α protein.This antibody also detects PI3K p55γ protein.

Molecular Weight: ~ 55, 85 kDa

Pathway: Phosphoinosotode Signaling, Protein Kinase C Signaling, Regμlation of Actin Dynamics, Warburg Effect, Regμlation of Microtubμle Dynamics, SAPK JNK Signaling Cascades, Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk, Mitochondrial Control of Apoptosis,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P27986/Q92569

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only