Product Description

PI3K p85 alpha Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB65973-50, MB65973-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3, 4, 5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4, 5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival. PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN. PI3Ks are composed of a catalytic subunit (p110) and a regμlatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regμlatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β. In contrast, p110γ associates with a p101 regμlatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins.

Category: Primary Antibody

Reactivity: Mouse, Rat

Host: Mouse

Applications: WB, IHC

Alternate Names: GRB1, Phosphatidylinositol 3-kinase regμlatory subunit alpha, PI3-kinase regμlatory subunit alpha, PI3K regμlatory subunit alpha, PtdIns-3-kinase regμlatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regμlatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regμlatory subunit p85-alpha

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant protein corresponding to mouse PI3K p85 alpha.

Dilution: WB (1/1000 - 1/2000), IHC (1/100 - 1/200)

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of PI3K p85 alpha protein.

Molecular Weight: ~ 85 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P26450

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only