PIAS1 Polyclonal Antibody-BS8489

PIAS1 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS8489-50, BS8489-100

Swiss-Prot: O75925

Host: Rabbit

PIAS1 Polyclonal Antibody-BS8489

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PIAS1 antibody. Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region., Immunofluorescence analysis of U2OS cells using PIAS1 antibody. Blue: DAPI for nuclear staining.

Alternative Name: PIAS1; DDXBP1; GBP; GU/RH-II; ZMIZ3

Immunogen: Recombinant fusion protein of human PIAS1(NP_057250.1).

Modification: Unmodified

Conjugate: Unconjugated