PIASx (G24) Polyclonal Antibody-BS1466

PIASx (G24) Polyclonal Antibody

Catalogue Numbers: BS1466-50, BS1466-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75928

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The IL-6-type family of cytokines, which includes IL-6 as well as a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the Stat family are known to be involved in this signal transduction pathway, undergoing phosphorylation, dimerization and translocation to the nucleus upon activation. PIAS 1, for protein inhibitor of activated Stat1 (also designated Gu/RNA helicase II binding protein), binds specifically to Stat1, blocking Stat1 DNA-binding activity and inhibiting Stat1-mediated gene activation. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PIAS2 (G24) polyclonal antibody detects endogenous levels of PIAS2 protein.

Extra Notes: Western blot (WB) analysis of PIASx (G24) pAb at 1:500 dilution
Lane1:MCF-7 whole cell lysate(40ug)
Lane2:HepG2 whole cell lysate(40ug)
Lane3:PC3 whole cell lysate(40ug)
Lane4:The Testis tissue lysate of Mouse(40ug)
Lane5:The Testis tissue lysate of Rat(40ug)

Bioworld Molecular Weight: ~ 68 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: E3 SUMO-protein ligase PIAS2; Protein inhibitor of activated STAT2; Protein inhibitor of activated STAT x; Msx-interacting zinc finger protein; Miz1; DAB2-interacting protein; DIP; Androgen receptor-interacting protein 3; ARIP3; PIAS-NY protein; PIAS

Immunogen: Synthetic peptide, corresponding to amino acids 1-50 of Human PIASx.

Conjugate: Unconjugated

Modification: Unmodified