Pig CNTF (Ciliary Neurotrophic Factor) ELISA Kit - ELK5967

Pig CNTF (Ciliary Neurotrophic Factor) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK5967-48, ELK5967-96, ELK5967-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: HCNTF

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Cytokine; Neuroscience;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig CNTF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig CNTF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig CNTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig CNTF in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O02732

Research Use Only