Pig FETUA (Fetuin A) ELISA Kit - ELK5746

Pig FETUA (Fetuin A) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK5746-48, ELK5746-96, ELK5746-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: HSGa; A2HS; AHS; FETUA; HSG-A2; Fetuin-A; Alpha 2-HS Glycoprotein; Fetuin A; Alpha-2-Z-Globulin; Ba-alpha-2-Glycoprotein; Alpha-2-Heremans Schmid Glycoprotein; aHSG

Assay Type: Sandwich

Sensitivity: 6.7 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Length: 3.5h

Research Areas: Infection immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig FETUA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig FETUA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig FETUA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig FETUA in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P29700

Research Use Only