Product Description

PIG3 (L265) Polyclonal Antibody

Catalogue Numbers: BS2085-50, BS2085-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q53FA7

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The PIG (p53-induced gene) gene family encodes redox-controlling proteins that are involved in p53 tumor suppressor activity. One member of the PIG gene family, p53-inducible gene 3 (PIG3), is a p53 responsive gene that maps, in humans, to chromosome 2p and encodes a protein with significant homology to oxidoreductases. Oxidoreductases are enzymes involved in cellular responses to oxidative stress and irradiation, and they influence the involvement of PIG3 in the metabolism of reactive oxygen species. PIG3 is localized to the cytoplasm and induced in primary, non-transformed, and transformed cell cultures after exposure to genotoxic agents. The induction of PIG3 is p53 dependent and occurs with delayed kinetics as compared with other p53 downstream targets. PIG3 may act with caspase-8 as a key regulatory element in p53-dependent transcriptional deregulation by triggering the caspase cascade and mitochondrial breakdown. PIG3 is highly up-regulated by p53 and may be useful for detecting transient activation of p53.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PIG3 (L265) polyclonal antibody detects endogenous levels of PIG3 protein.

Extra Notes: Western blot (WB) analysis of PIG3 (L265) polyclonal antibody at 1:500 dilution
Lane1:HEK293T whole cell lysate
Lane2:Raw264.7 whole cell lysate
Lane3:H9C2 whole cell lysate

Bioworld Molecular Weight: ~ 36 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Quinone oxidoreductase PIG3; Tumor protein p53-inducible protein 3; p53-induced gene 3 protein; TP53I3; PIG3

Immunogen: Synthetic peptide, corresponding to amino acids 230-286 of Human PIG3.

Conjugate: Unconjugated

Modification: Unmodified