Product Description

PIGW Polyclonal Antibody

Catalogue Numbers: BS60200-50, BS60200-100

Sizes: 50µl, 100µl

Alternative Name: Phosphatidylinositol-glycan biosynthesis class W protein; PIG-W

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q7Z7B1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Phosphatidylinositol-glycans (PIGs) are multi-pass transmembrane proteins that localize to the endoplasmic reticulum. PIGs exhibit various functions, but all are crucial for the biosynthesis of the glycosylphosphatidylinositol (GPI)-anchor. Some PIG proteins are components of the GPI transamidase complex and play a role in the recognition of either the GPI attachment signal or the lipid portion of GPI. Other PIGs belong to the glycosyltransferase complex and function in the transfer of N-acetylglucosamine (GlcNAc) to phosphatidylinositol (PI). A variety of other PIGs play distinct roles in GPI synthesis including mannosylation of the GPI-anchor. PIG-W (Phosphatidylinositol-glycan biosynthesis class W protein) is a 504 amino acid multi-pass membrane protein that functions in the third step of GPI biosynthesis and acylates the inositol ring of phosphatidylinositol.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PIGW polyclonal antibody detects endogenous levels of PIGW protein.

Bioworld Molecular Weight: ~ 57 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PIGW polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human PIGW.

Conjugate: Unconjugated

Modification: Unmodified