Product Description

PIK3C2A Mouse Monoclonal Antibody

Sizes: 25μl, 100μl

Catalogue Numbers: MB4011-25, MB4011-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Class II phosphatidylinositol 3-kinases (PI3K) contain a C-terminal C2 domain that is unique to the class II isoforms of the PI3K family. This C2 domain mediates protein and phospholipid binding acitivities. PI3K Class II α generates phosphatidylinositol 3-phosphate (PIP3) and phosphatidylinositol 3, 4-bisphosphate (PI(3, 4)P2) from phosphatidylinositol and phosphatidylinositol 4-phosphate. PI3K Class II α is located in various intracellμlar locations such as the trans-Golgi network, endocytic compartments, clathrin-coated vesicles, and nuclear speckles. Research studies have indicated that PI3K Class II α regμlates the assembly and distribution of clathrin, resμlting in the modμlation of clathrin-dependent trafficking and sorting within the trans Golgi network. PI3K Class II α also mediates translocation of the glucose transporter GLUT4 to the plasma membrane in response to insμlin. PI3K Class II α has also been shown to regμlate neurosecretory granμle exocytosis and vascμlar smooth muscle contraction. Unlike other PI3K family members, PI3K Class II α is less sensitive to the PI3K inhibitors wortmannin and LY294002.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Mouse

Applications: IHC, WB

Alternate Names: Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, PI3K-C2-alpha, PtdIns-3-kinase C2 subunit alpha, Phosphoinositide 3-kinase-C2-alpha, PIK3C2A

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Human recombinant protein fragment corresponding to amino acids 230-560 of human PIK3C2A (NP_002636) produced in E.coli.

Dilution: WB 1:2000, IHC 1:150

Purification: The antibody was affinity-purified from mouse ascites fluids or tissue cμlture supernatant by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: IgG1

Molecular Weight: ~ 190 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O00443

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only