Product Description

PIK3C2A Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS71277-50, BS71277-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O00443

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Background: The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PIK3C2A polyclonal antibody detects endogenous levels of PIK3C2A protein.

Bioworld Molecular Weight: ~ 191 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cells, using PIK3C2A antibody., Immunohistochemistry of paraffin-embedded mouse heart using PIK3C2A antibody at dilution of 1:100 (x40 lens)., Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug PIK3C2A antibody.

Alternative Name: CPK; PI3-K-C2A; PI3-K-C2(ALPHA);

Immunogen: Recombinant protein of human PIK3C2A.

Conjugate: Unconjugated

Modification: Unmodified