Product Description

PIM1 (I302) Polyclonal Antibody

Catalogue Numbers: BS2988-50, BS2988-100

Sizes: 50µl, 100µl

Alternative Name: Serine/threonine-protein kinase pim-1; Pim1; Pim-1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P11309

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Pim-1 is a serine/threonine kinase that cooperates with c-Myc in lymphoid cell transformation. The expression of Pim-1 increases during the progression from early to late G1, remaining high at the G1/S boundary and G2 phases of the cell cycle. Pim-1 is regulated at both the transcriptional and translational level, and it has been shown to be induced by IL-2 stimulation. Pim-1 also plays a role in T cell differentiation, and it has been shown to stimulate c-Myc-mediated apoptosis upstream of caspase-3-like proteases.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PIM1 (I302) polyclonal antibody detects endogenous levels of PIM1 protein.

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PIM1 (I302) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 271-320 of Human Pim-1.

Conjugate: Unconjugated

Modification: Unmodified