PIM1 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB11820-50, MB11820-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P11309
Host: Rabbit
Reactivity: Human
Applications: WB, IHC-P
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100
Background: Pim1 a proto-oncogene serine/threonine kinase involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerced by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 36 kDa; Observed MW: 36 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PIM1 in Hela, A549, HL-60 lysates using PIM1 antibody., Immunohistochemistry analysis of paraffin-embedded Human tonsil using PIM1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Alternative Name: PIM1; Serine/threonine-protein kinase pim-1
Immunogen: A synthetic peptide of human PIM1
Conjugate: Unconjugated
Modification: Unmodified