Product Description

PIN1 (phospho-S16) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4860-50, BS4860-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: NIMA was originally shown in Aspergillus nidμlans to be necessary for entry into mitosis. NIMA-related mammalian proteins have since been identified as Nek1, Nek2 and Nek3. High expression of Nek1 is seen in male and female germ cell lines of mouse. Nek2 is the closest known mammalian relative to NIMA. Like NIMA, Nek2 expression peaks at the G2 to M phase transition. Pin1 was originally identified as a NIMA-interacting protein. Pin1 is a peptidyl-prolyl cis/trans isomerase (PPIase), which specifically binds to phosphoserine-proline or phosphothreonine-proline bonds in mitotic phosphoproteins. While previously identified PPIases have been shown to be involved in protein folding, assembly and transport, Pin1 is the first PPIase to be identified as a required protein for cell viability.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-trans isomerase Pin1, PPIase Pin1, Rotamase Pin1, PIN1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human PIN1 around the phosphorylation site of Serine 16.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PIN1 polyclonal antibody detects endogenous levels of PIN1 protein.

Molecular Weight: ~ 18 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q13526

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only