Product Description

PINK1 (Phospho-S228) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64545-50, BS64545-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9BXM7

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB:1:500~1:1000

Background: PTEN induced putative kinase 1, PINK1, is a mitochondrial serine/threonine kinase involved in the normal function and integrity of mitochondria, as well as in reduction of cytochrome c release from mitochondria. PINK1 phosphorylates Parkin and promotes its translocation to mitochondria. Research studies have shown that mutations in PINK1 are linked to autosomal recessive early onset Parkinson’s disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of α-synuclein, as well as proteasome dysfunction.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PINK1 (Phospho-S228) polyclonal antibody detects endogenous levels of PINK1 protein only when phosphorylated at Ser228.

Bioworld Molecular Weight: ~ 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot (WB) analysis of PINK1 (Phospho-S228) polyclonal antibody at 1:500 dilution
Lane A: The Testis tissue lysate of Mouse
Lane B: The Testis tissue lysate of Rat
Lane C: HEK293T whole cell lysate
Lane D: MCF-7 whole cell lysate
Lane E: U-87MG whole cell lysate

Alternative Name: Serine/threonine-protein kinase PINK1, mitochondrial; BRPK; PTEN-induced putative kinase protein 1; PINK1

Immunogen: Synthetic phosphopeptide derived from human PINK1 around the phosphorylation site of Serine 228.

Conjugate: Unconjugated

Modification: Phosphorylation