PIP Monoclonal Antibody-MB66213

PIP Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66213-50, MB66213-100

Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Swiss-Prot: P12273

Host: Mouse

Reactivity: Human

Applications: IHC

All Applications: IHC (1/100 - 1/300)

Background: Gross cystic disease of the breast is a benign premenopausal disorder in which cysts are a predominant pathological lesion. These cysts appear to be formed from excessive aporcine cystic secretions. This fluid is composed of several glycoproteins including a unique 15kD monomer protein, PIP. Cytosolic analysis of normal tissue specimens from all major organs has demonstrated PIP in aporcine epithelia, lacrimal, ceruminous and Moll’s glands and in numerous serous cells of the submandibular, tracheal, bronchial, sublingual and minor salivary glands. PIP and prostate specific antigen are co-expressed in androgen receptor-positive breast tumors.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of PIP protein.

Bioworld Molecular Weight: ~ 16 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemical analysis of PIP staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of PIP staining in human salivary gland formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of PIP staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Alternative Name: GCDFP15; GPIP4; Prolactin-inducible protein; Gross cystic disease fluid protein 15; GCDFP-15; Prolactin-induced protein; Secretory actin-binding protein; SABP; gp17

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human PIP. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified