Product Description

PIP5K (Q95) Polyclonal Antibody

Catalogue Numbers: BS2676-50, BS2676-100

Sizes: 50µl, 100µl

Alternative Name: 1-phosphatidylinositol 3-phosphate 5-kinase; Phosphatidylinositol 3-phosphate 5-kinase; FYVE finger-containing phosphoinositide kinase; PIKfyve; Phosphatidylinositol 3-phosphate 5-kinase type III; PIPkin-III; Type III PIP kinase; PIKFYVE; KIAA0981; PIP5K3

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y2I7

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:1000~1:2000

Background: Phosphatidylinositol-4-phosphate-5-kinase (PIPK) synthesizes phosphatidylinositol-4,5-bisphosphate, which regulates various processes including cell proliferation, survival, membrane trafficking and cytoskeletal organization. The PIPK family is divided into type I, type II and type III. Each type of the PIPK family phosphorylates distinct substrates. They contain an activation loop, which determines their enzymatic specificity and subcellular targeting. The phosphatidylinositol-4-phosphate-5-kinase type I consists of three members, PIPK I α, β and γ, which are characterized by phosphorylating PI4P on the 5-hydroxyl. PIPK I α, designated PIPK I β in mouse, is expressed in brain tissue. PIPK I β, designated PIPK I α in mouse, is also called STM7. PIPK I γ has two variants produced by alternative splicing which are expressed in lung, brain and kidneys.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PIP5K (Q95) pAb detects endogenous levels of PIP5K protein.

Bioworld Molecular Weight: ~ 237 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PIP5K (Q95) pAb at 1:1000 dilution
Lane 1: A2780 whole cell lysate(40ug)
Lane 2: The Testis tissue lysate of Rat(40ug)
Lane 3: The Testis tissue lysate of Mouse(30ug)
Lane 4: Hela whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 65-125 of Human PIP5K.

Conjugate: Unconjugated

Modification: Unmodified