Product Description

PIP5K1B Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS72368-50, BS72368-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O14986

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4P/PI4P to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5P2/PIP2, a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility (By similarity. PtdIns(4,5P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers: inositol 1,4,5-trisphosphate (IP3, diacylglycerol (DAG or phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5P3/PIP3 (By similarity. Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of phospholipase PLD2. Together with PIP5K1A, is required, after stimulation by G-protein coupled receptors, for the synthesis of IP3 that will induce stable platelet adhesion (By similarity.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 71kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using PIP5K1B antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s., Immunofluorescence analysis of U2OS cells using PIP5K1B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: PIP5K1B; MSS4; STM7

Immunogen: Recombinant fusion protein of human PIP5K1B(NP_003549.1).

Conjugate: Unconjugated

Modification: Unmodified