Product Description

PITPNB Polyclonal Antibody

Catalogue Numbers: BS5858-50, BS5858-100

Sizes: 50µl, 100µl

Alternative Name: Phosphatidylinositol transfer protein beta isoform; PI-TP-beta; PtdIns transfer protein beta; PtdInsTP beta; PITPβ; PI-TPβ; PI-TP-β

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P48739

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The lipid binding proteins known as phosphatidylinositol transfer proteins (PITP) facilitate the formation of phosphatidylinositol derived second messenger molecules, which are related to the phospholipase C and phosphoinositide 3-kinase pathways. PITP are ubiquitously expressed proteins that transfer phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes enriched in PI or PC to membranes that are deficient in PI or PC. PITP mobilizes PI from the endoplasmic recticulum and regulates the release of PI from stored vesicles in the Golgi network. In mammalian cells, three smaller forms of soluble PITP are present, designated PITPα, PITPβ and retinal degeneration B (rdgB) beta. PITPβ is a 271 amino acid protein that is widely expressed in various tissues. Though required for Golgi targeting, constitutive phosphorylation of Ser-262 has no effect on phospholipid transfer activity. There are two isoforms of PITPβ that are produced as a result of alternative splicing events.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PITPNB polyclonal antibody detects endogenous levels of PITPNB protein.

Bioworld Molecular Weight: ~ 32 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PITPNB polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: Rat heart tissue lysate

Immunogen: Synthetic peptide, corresponding to amino acids 226-271 of Human PITPNB.

Conjugate: Unconjugated

Modification: Unmodified