Product Description

PKAα/β cat (K17) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS2648-50, BS2648-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The second messenger cyclic AMP (cAMP) mediates diverse cellμlar responses to external signals such as proliferation, ion transport, regμlation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regμlatory subunits of the tetrameric PKA holoenzyme resμlting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels resμlts from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: cAMP-dependent protein kinase catalytic subunit alpha, PKA C-alpha, PRKACA, PKACA, PKA Ca, cAMP-dependent protein kinase catalytic subunit beta, PKA C-beta, PRKACB, PKACB, PKA Cb,

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to the N-terminus of Human PKAα.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PKAα/β cat (K17) polyclonal antibody detects endogenous levels of PKAα/β protein.

Molecular Weight: ~ 40, 42 kDa

Pathway: Regμlation of Actin Dynamics, Regμlation of Microtubμle Dynamics, Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk, Mitochondrial Control of Apoptosis,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P17612/P22694

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only