PKA 2β Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91078-50, BS91078-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P31323(Human) P31324(Mouse) P12369(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IP, ICC/IF IHC FC
All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: The second messenger cyclic AMP mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. One of several regulatory subunits, p-PKA IIβ reg (cAMP-dependent protein kinase type II-beta regulatory subunit), also known as PRKAR2B, is a 418 amino acid protein that is phosphorylated by the activated catalytic chain. p-PKA IIβ reg knockout mice exhibit diminished white adipose tissue and were protected against diet-induced obesity and fatty livers,as well as markedly reduced leptin mRNA. Also playing a role in the immune response, p-PKA IIβ reg suppresses CREB transcriptional activity and down-regulates IL-2 production in T-lymphocytes.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: PKA 2β polyclonal antibody detects endogenous levels of PKA 2β protein.
Bioworld Molecular Weight: 46 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: ICC staining PKA 2 beta (regulatory subunit) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining PKA 2 beta (regulatory subunit) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: PRKAR2B, AI451071, AW061005, cAMP dependent protein kinase type II beta regulatory chain, cAMP dependent protein kinase type II beta regulatory subunit, cAMP-dependent protein kinase type II-beta regulatory subunit, H RG363E19.2, KAP3, MGC116401, Pkarb2, PRKAR 2, PRKAR2, PRKAR2B, Protein kinase cAMP dependent regulatory type II beta, RATDNA, RII beta, RII(beta), RIIbeta, WUGSC:H RG363E19.2,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified