PKA R2 (Phospho-S99) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS94040-50, BS94040-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P13861(Human) P12367(Mouse) P12368(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat, Pig
Applications: WB, ICC/IF, IHC, IP,
All Applications: WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200
Background: The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: PKA R2 (Phospho-S99) polyclonal antibody detects endogenous levels of PKA R2 protein only when phosphorylated at S99.
Bioworld Molecular Weight: 51 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: ICC staining phospho-PKA R2 (S99) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining phospho-PKA R2 (S99) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: PRKAR2A, cAMP dependent protein kinase regulatory subunit alpha 2, cAMP dependent protein kinase regulatory subunit RII alpha, cAMP dependent protein kinase type II alpha regulatory chain, cAMP dependent protein kinase type II alpha regulatory subunit, cAMP-dependent protein kinase type II-alpha regulatory subunit, KAP2, KAP2, MGC3606, PKR 2, PKR2, PRKA R2, PRKAR 2, PRKAR2, PRKAR2A, Protein kinase A RII alpha subunit, Protein kinase cAMP dependent regulatory type II alpha,
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser99 of human PKA R2
Conjugate: Unconjugated
Modification: Phosphorylation